In 1993, scientists at Rutgers
University invented a new type of polyploid
oyster — tetraploids — which contain
four complete sets of chromosomes (4C). Unlike
triploid shellfish, tetraploids are fully fertile.
Using
a proprietary, patented, process, 4Cs
Breeding Technologies Inc.
produces and nurtures tetraploid brood
stock.
Males are identified from
our select brood stock lines and sacrificed
for their sperm. The sperm from our tetraploid
males is naturally diploid (2C), instead
of haploid (1C) as it would be from typical
diploid males.
The diploid sperm is added to
haploid eggs from the customer's normal diploid
brood stock. No induction treatment is used.
The resulting zygotes are genetically triploid,
with two sets of chromosomes contributed by
the sperm and one set contributed by the egg.
4Cs Natural Triploids have
all of the advantages of induced triploids,
in the hatchery and in
the field, with none
of the drawbacks:
4Cs Natural Triploids are
genetically 100% triploid.
4Cs Natural Triploid larvae
suffer no mortality from treatment effects,
because they are derived naturally by
genetic mating.
4Cs Natural Triploid eyed
larvae have a dark color, large size and
high setting efficiency.
Licensing through 4Cs provides
brood stock for spawning and we can provide
technical assistance in the hatchery. Research
and development is underway to develop protocols
for distribution of cryopreserved sperm from
tetraploids.
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